![]() ![]() Keywords:ALTERNATIVE ENERGY SOURCE, RENEWABLE ENERGY, CLEAN ENERGY, SOLAR ENERGY, RENEWABLE FUEL, WASTE TO ENERGY, CO 2 MITIGATION,Īn interdisciplinary student team will first use microalgae in a photobioreactor to efficiently produce oil using CO 2 and solar light as an energy source. ![]() Hydrogen from the hollow-fiber bioreactor was directly injected into a small fuel cell, and shown to be capable of generating enough electricity to power a small motor. aerogenes from glycerol was observed for 3 days at a maximum rate of 30 mL per hour (mL Hydrogen production in the hollow-fiber bioreactor by E. The assessment of H2 and ethanol-producing activity by bacteria in the hollow-fiber bioreactor in the presence of 2% glycerol (v/v) was made. Glycerol uptake efficiency by bacteria in the bioreactor was found to be 90% for 7 days. Used growth medium was returned to the bioreactor medium reservoir, to create a close system in which it was possible to measure glycerol uptake and ethanol production by bacterial cells. Bacterial cells were readily adsorbed to the outer surface of the hollow fibers, and the cells consumed glycerol. The bioreactor was designed in a way that the glycerol diluted in growth medium was pumped from the outside of the fibers into the lumen (inside). A lab-scale hollow-fiber bioreactor for conversion of glycerol into H2 and ethanol was constructed. The yield of H2 from glycerol (0.9 mol/mol) was relatively high in batch culture (in test tubes). Highest H2 and ethanol production rates were observed under 2% glycerol, volume per volume (v/v), on a synthetic medium in test tubes. Next, glycerol was used as a substrate for making H2 and ethanol by bacterium Enterobacter aerogenes in batch culture (test tubes), and in hollow-fiber bioreactor. Algal oil was converted into biodiesel by transesterification with glycerol as a by-product. Oil content in the algal cells in the bioreactor was found to be 10%. The amount of oily material was expressed as a percentage of algal dry weight. Algal biomass was harvested at OD665 ~ 0.35 corresponding to a chlorophyll concentration of ~ 3 μg per ml, and concentrated by sedimentation with subsequent drying under 70◦C. Continuous light was provided by cool white fluorescent lamps (136 - 186 μmol Photobioreactor was run in a batch mode for two weeks at room temperature. The gas mixture (5% CO2 and air) flowed up the top of the PVC tubes from the bottom as large gas bubbles. A 100 L photobioreactor for biodiesel generation from microalga Chlorella vulgaris was constructed from two parallel clear PVC 10 feet tubes (6’ diameter) with a small slope (10%). ![]()
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